[A study of a derivative of Taq DNA polymerase].
Yi Chuan Xue Bao
; 26(2): 179-85, 1999.
Article
em Zh
| MEDLINE
| ID: mdl-10375865
ABSTRACT
Using the method of double primer oligonucleotide-mediated mutagenesis, the high expression plasmid of TaqND236, a derivative of Taq DNA polymerase, was constructed. To determine the frameshift mutation frequency of the in vitro DNA synthesis, we constructed a Gapped-DNA system using the pFDPM118 (a mutant of pUC118 with a -1 frameshift mutation on the lacZ gene) as template. By calculating the ratio of blue and white colonies on the X-gal plate after transforming E. coli TG1, the frameshift mutation frequency of Taq and TaqND236 was measured. It was found that the replication fidelity of the deleted Taq-TaqND236 increased more than 10 folds.
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Base de dados:
MEDLINE
Assunto principal:
Taq Polimerase
Idioma:
Zh
Ano de publicação:
1999
Tipo de documento:
Article