Monitoring rotavirus environmental contamination in a pediatric unit using polymerase chain reaction.
Infect Control Hosp Epidemiol
; 20(6): 432-4, 1999 Jun.
Article
em En
| MEDLINE
| ID: mdl-10395149
ABSTRACT
Rotavirus environmental contamination in a pediatric unit was investigated. Surfaces were swabbed, then viruses eluted, ultracentrifuged, and detected by polymerase chain reaction (PCR) amplification. Of 55 samples, 25 (46%) tested positive. Rotavirus RNA was more prevalent on surfaces in direct contact with children (thermometers and play mats) than on other environmental surfaces (washbasins, door handles, etc). PCR has proved useful for monitoring rotavirus environmental contamination.
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Base de dados:
MEDLINE
Assunto principal:
RNA Viral
/
Unidades de Terapia Intensiva Pediátrica
/
Monitoramento Ambiental
/
Contaminação de Equipamentos
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Rotavirus
/
Microbiologia Ambiental
Limite:
Child
/
Humans
/
Newborn
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article