Analysis of fliC variation among clinical isolates of Burkholderia cepacia.
J Med Microbiol
; 48(7): 657-662, 1999 Jul.
Article
em En
| MEDLINE
| ID: mdl-10403416
ABSTRACT
PCR and restriction fragment length polymorphism (RFLP) typing of flagellin genes (fliC) from 57 clinical isolates of Burkholderia cepacia indicated that only type 11 flagellins were present. Twenty-two isolates previously identified as the epidemic UK cystic fibrosis strain were indistinguishable by this method, as were 11 isolates from a pseudo-outbreak in Senegal. Other clinical isolates, including 19 from disparate sources in Malaysia, were separated into nine fliC RFLP groups, exhibiting a large degree of divergence. When isolates were indistinguishable by fliC genotyping, their similarity was confirmed by whole genome macro-restriction analysis with pulsed-field gel electrophoresis following XbaI digestion. The variation in fliC sequences of B. cepacia was far greater than that with B. pseudomallei, supporting the view that 'B. cepacia', as currently defined, may comprise several different genomic species.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Variação Genética
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Burkholderia cepacia
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Infecções por Burkholderia
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Fibrose Cística
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Flagelina
Limite:
Humans
País como assunto:
Africa
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Asia
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Europa
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article