Transport of genistein-7-glucoside by human intestinal CACO-2 cells: potential role for MRP2.
Res Commun Mol Pathol Pharmacol
; 103(1): 45-56, 1999 Jan.
Article
em En
| MEDLINE
| ID: mdl-10440570
ABSTRACT
This study demonstrated rapid apical to basolateral absorption of genistein across Caco-2 cell monolayers with a P(app) of 20.0+/-0.8 x 10(-6)cm/sec. In contrast, genistein's main dietary source, genistein-7-glucoside (genistin), was not absorbed. In fact, genistin demonstrated basolateral to apical efflux of 1.28+/-0.10 x 10(-6) cm/sec, which exceeded the flux of the paracellular transport marker mannitol by 6-fold. Although genistin hydrolysis to genistein occurred in the preparation, it did not contribute significantly to these observations. In experiments trying to define the efflux mechanism for genistin, MK-571 reduced the efflux by 87% to 0.19+/-0.02 x 10(-6) cm/sec, implying transport by the polarized efflux pump MRP2.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Transporte
/
Isoflavonas
Limite:
Humans
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article