GLUT2 and glucokinase expression is coordinately regulated by sulfonylurea.
Mol Cell Endocrinol
; 153(1-2): 155-61, 1999 Jul 20.
Article
em En
| MEDLINE
| ID: mdl-10459863
ABSTRACT
In the present study we examined the effect of sulfonylurea on the expression of the glucose transporter GLUT2 and the glucose phosphorylating enzyme Glucokinase (GK) in betaTC6-F7 cells; furthermore, we studied the modifications induced by sulfonylurea on glucose-responsiveness and -sensitivity. Results demonstrate that sulfonylurea increases GLUT2 and GK mRNA expression after 24 h in a dose-dependent manner. On the contrary, after 48 and 72 h a time-dependent reduction of both GLUT2 and GK mRNA occurs. GLUT2 and GK protein expression follow the same modifications. Therefore, GLUT2 and GK are coordinately regulated by sulfonylurea, probably by a common mechanism. Glucose-induced insulin release is increased by sulfonylurea as well as glucose sensitivity. Our study suggests that short-term effect of sulfonylurea increases while long-term effect reduces the expression of glucose sensing elements. The long-term inhibitory effect on glucose sensing elements would explain the reduced insulin secretion occurring after chronic sulfonylurea treatment.
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Base de dados:
MEDLINE
Assunto principal:
Compostos de Sulfonilureia
/
Proteínas de Transporte de Monossacarídeos
/
Regulação da Expressão Gênica
/
Glucoquinase
/
Hipoglicemiantes
Limite:
Animals
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article