Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis.
Antimicrob Agents Chemother
; 43(10): 2550-4, 1999 Oct.
Article
em En
| MEDLINE
| ID: mdl-10508043
ABSTRACT
We applied double gradient-denaturing gradient gel electrophoresis (DG-DGGE) for the rapid detection of rifampin (RMP) resistance from rpoB PCR products of Mycobacterium tuberculosis isolates and clinical samples. The results of this method were fully concordant with those of DNA sequencing and susceptibility testing analyses. DG-DGGE is a valid alternative to the other methods of detecting mutations for predicting RMP resistance.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Rifampina
/
Eletroforese em Gel de Poliacrilamida
/
Antibióticos Antituberculose
/
Mycobacterium tuberculosis
Tipo de estudo:
Diagnostic_studies
Limite:
Adult
/
Aged
/
Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article