Biosynthesis and turnover of anandamide and other N-acylethanolamines in peritoneal macrophages.
FEBS Lett
; 459(1): 123-7, 1999 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-10508930
ABSTRACT
Polyunsaturated N-acylethanolamines (NAEs), including anandamide (204n-6 NAE), elicit a variety of biological effects through cannabinoid receptors, whereas saturated and monounsaturated NAEs are inactive. Arachidonic acid mobilization induced by treatment of intact mouse peritoneal macrophages with Ca2+ ionophore A23187 had no effect on the production of NAE or its precursor N-acylphosphatidylethanolamine (N-acyl PE). Addition of exogenous ethanolamine resulted in enhanced NAE synthesis by its N-acylation with endogenous fatty acids, but this pathway was not selective for arachidonic acid. Incorporation of (18)O from H2 (18)O-containing media into the amide carbonyls of both NAE and N-acyl PE demonstrated a rapid, constitutive turnover of both lipids.
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Base de dados:
MEDLINE
Assunto principal:
Ácidos Araquidônicos
/
Macrófagos Peritoneais
/
Etanolaminas
Limite:
Animals
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article