Microtubule growth activates Rac1 to promote lamellipodial protrusion in fibroblasts.
Nat Cell Biol
; 1(1): 45-50, 1999 May.
Article
em En
| MEDLINE
| ID: mdl-10559863
ABSTRACT
Microtubules are involved in actin-based protrusion at the leading-edge lamellipodia of migrating fibroblasts. Here we show that the growth of microtubules induced in fibroblasts by removal of the microtubule destabilizer nocodazole activates Rac1 GTPase, leading to the polymerization of actin in lamellipodial protrusions. Lamellipodial protrusions are also activated by the rapid growth of a disorganized array of very short microtubules induced by the microtubule-stabilizing drug taxol. Thus, neither microtubule shortening nor long-range microtubule-based intracellular transport is required for activating protrusion. We suggest that the growth phase of microtubule dynamic instability at leading-edge lamellipodia locally activates Rac1 to drive actin polymerization and lamellipodial protrusion required for cell migration.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas rac1 de Ligação ao GTP
/
Microtúbulos
Limite:
Animals
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article