G1-phase and B-type cyclins exclude the DNA-replication factor Mcm4 from the nucleus.
Nat Cell Biol
; 1(7): 415-22, 1999 Nov.
Article
em En
| MEDLINE
| ID: mdl-10559985
ABSTRACT
Cyclin-dependent kinases (CDKs) activate the firing of replication origins during the S phase of the cell cycle. They also block re-initiation of DNA replication within a single cell cycle, by preventing the assembly of prereplicative complexes at origins. We show here that, in budding yeast, CDKs exclude the essential prereplicative-complex component Mcm4 from the nucleus. Although origin firing can be triggered by the B-type cyclins only, both G1-phase and B-type cyclins cause exit of Mcm4 from the nucleus. These results suggest that G1 cyclins may diminish the cell's capacity to assemble prereplicative complexes before B-type cyclins trigger origin firing during S phase.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Fúngicas
/
Núcleo Celular
/
Fase G1
/
Fase S
/
Ciclinas
/
Quinases Ciclina-Dependentes
/
Proteínas de Ciclo Celular
/
Proteínas de Saccharomyces cerevisiae
/
Proteínas de Ligação a DNA
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article