Your browser doesn't support javascript.
loading
Development of class-switched, affinity-matured monoclonal antibodies following a 7-day immunization schedule.
Bynum, J; Andrews, J L; Ellis, B; Kull, F C; Austin, E A; Kilpatrick, K E.
Afiliação
  • Bynum J; Department of Molecular Sciences, Glaxo Wellcome, Research Triangle Park, NC 27709, USA.
Hybridoma ; 18(5): 407-11, 1999 Oct.
Article em En | MEDLINE | ID: mdl-10600027
Previously we reported making high-affinity monoclonal antibodies (MAbs) 13 days after the onset of Repetitive Immunizations Multiple Sites (RIMMS) strategy. The Ig subclass variety and affinity of these antibodies suggested that maturational processes had already begun within draining lymph nodes. We now demonstrate that this diversity can in fact be captured as early as Day 7. In the work reported here, somatic fusion of immune lymphocytes isolated from peripheral lymph nodes resulted in the isolation of affinity-matured MAbs reactive with cytosine deaminase. This model further demonstrates and substantiates at a cellular level the rapid development and maturation of T-cell-dependent B-cell responses occurring within draining lymph nodes following antigen challenge.
Assuntos
Buscar no Google
Base de dados: MEDLINE Assunto principal: Anticorpos Monoclonais Limite: Animals Idioma: En Ano de publicação: 1999 Tipo de documento: Article
Buscar no Google
Base de dados: MEDLINE Assunto principal: Anticorpos Monoclonais Limite: Animals Idioma: En Ano de publicação: 1999 Tipo de documento: Article