Cholesterol binds to synaptophysin and is required for biogenesis of synaptic vesicles.
Nat Cell Biol
; 2(1): 42-9, 2000 Jan.
Article
em En
| MEDLINE
| ID: mdl-10620806
ABSTRACT
Here, to study lipid-protein interactions that contribute to the biogenesis of regulated secretory vesicles, we have developed new approaches by which to label proteins in vivo, using photoactivatable cholesterol and glycerophospholipids. We identify synaptophysin as a major specifically cholesterol-binding protein in PC12 cells and brain synaptic vesicles. Limited cholesterol depletion, which has little effect on total endocytic activity, blocks the biogenesis of synaptic-like microvesicles (SLMVs) from the plasma membrane. We propose that specific interactions between cholesterol and SLMV membrane proteins, such as synaptophysin, contribute to both the segregation of SLMV membrane constituents from plasma-membrane constituents, and the induction of synaptic-vesicle curvature.
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Base de dados:
MEDLINE
Assunto principal:
Vesículas Sinápticas
/
Colesterol
/
Sinaptofisina
/
Exocitose
/
Neurônios
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article