Molecular cloning, expression and immunological characterisation of Lol p 5C, a novel allergen isoform of rye grass pollen demonstrating high IgE reactivity.
FEBS Lett
; 462(3): 435-41, 1999 Dec 03.
Article
em En
| MEDLINE
| ID: mdl-10622741
ABSTRACT
A novel isoform of a major rye grass pollen allergen Lol p 5 was isolated from a cDNA expression library. The new isoform, Lol p 5C, shares 95% amino acid sequence identity with Lol p 5A. Both isoforms demonstrated shared antigenic activity but different allergenic activities. Recombinant Lol p 5C demonstrated 100% IgE reactivity in 22 rye grass pollen sensitive patients. In comparison, recombinant Lol p 5A showed IgE reactivity in less than 64% of the patients. Therefore, Lol p 5C represents a novel and highly IgE-reactive isoform allergen of rye grass pollen.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Imunoglobulina E
/
Alérgenos
Limite:
Humans
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article