Humoral immune responses among mucosal and cutaneous leishmaniasis patients caused by Leishmania braziliensis.
J Parasitol
; 85(6): 1076-83, 1999 Dec.
Article
em En
| MEDLINE
| ID: mdl-10647040
ABSTRACT
Mucosal leishmaniasis is arguably the most morbid sequelae of cutaneous leishmaniasis. The importance of early diagnosis for effective therapy, coupled with the difficulty of diagnosing the disease parasitologically, prompted this investigation of humoral immune markers of mucosal disease. Promastigote soluble antigens of Leishmania braziliensis, isolated from cutaneous and mucosal lesions, were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis; antigens were identified by immunoblotting with parasite-specific IgG antibody-positive sera of patients with mucosal disease (n = 18) and cutaneous disease (n = 23). For antigens of the cutaneous parasite WR 2095, mucosal sera generally reacted intensely to antigens of 75, 66, and 45 kDa and weakly to 48-50-kDa antigens, whereas cutaneous sera generally detected weakly the first 3 antigens and intensely the latter doublet. The data suggest that the transition from the cutaneous antigenic profile to a mucosal antigenic profile could be used to predict mucosal disease in approximately half of mucosal patients. An additional finding was that antibodies present in the sera of patients with mucosal disease labeled a 66-kDa peptide of normal human lip mucosa more intensely than did cutaneous sera. Autoimmune processes stimulated by the reaction of IgG, originally directed against the 66-kDa of L. braziliensis, to the 66-kDa antigen of mucosal tissue may contribute to the clinical presentation of mucosal leishmaniasis.
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Base de dados:
MEDLINE
Assunto principal:
Leishmania braziliensis
/
Anticorpos Antiprotozoários
/
Leishmaniose Mucocutânea
/
Leishmaniose Cutânea
Tipo de estudo:
Prognostic_studies
/
Screening_studies
Limite:
Adolescent
/
Adult
/
Aged
/
Animals
/
Female
/
Humans
/
Male
/
Middle aged
País como assunto:
America do sul
/
Brasil
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article