Periovulatory human oocytes, cumulus cells, and ovarian leukocytes express type 1 but not type 2 11beta-hydroxysteroid dehydrogenase RNA.
Fertil Steril
; 73(4): 825-30, 2000 Apr.
Article
em En
| MEDLINE
| ID: mdl-10731548
ABSTRACT
OBJECTIVE:
To further elucidate cortisol metabolism in the follicular microenvironment at the time of oocyte retrieval, the presence of 11beta-hydroxysteroid dehydrogenase (HSD) messenger (m)RNA transcripts in oocytes; cumulus cells; granulosa cells; and CD45(+), CD15(+) leukocytes was assessed semiquantitatively.DESIGN:
Controlled study using semiquantitative assessment of 11beta-HSD mRNA.SETTING:
University IVF center. PATIENT(S) Twenty-six patients undergoing controlled ovarian hyperstimulation for assisted conception. INTERVENTION(S) Metaphase II oocytes; cumulus cells; granulosa cells, and CD45(+), CD15(+) leukocytes from individual follicular fluid aspirates. MAIN OUTCOMEMEASURES:
Semiquantitative analysis of PCR products after total RNA extraction and complementary DNA synthesis. RESULT(S) Periovulatory human oocytes; cumulus cells; CD45(+), CD15(+) leukocytes; and granulosa cells consistently express type 1 but not type 2 11beta-HSD mRNA. Expression of mRNA is greatest in cumulus cells. Type 1 11beta-HSD mRNA expression varies considerably in all cell types and among individual follicles and patients. CONCLUSION(S) These studies of mRNA expression suggest that the enzymes present both in and around the periovulatory oocyte will favor a high-cortisol environment.
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Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Ovário
/
Hidroxiesteroide Desidrogenases
/
Leucócitos
Tipo de estudo:
Prognostic_studies
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article