Capillary electrophoresis of DNA damage after irradiation: apoptosis and necrosis.
J Chromatogr A
; 871(1-2): 321-30, 2000 Feb 25.
Article
em En
| MEDLINE
| ID: mdl-10735312
ABSTRACT
Apoptosis is a type of cellular death but also directly regulates tumorigenesis through different gene expression. This phenomenon is often used as end-point in studies of radio- and chemosensitivity of cancer cells. Restriction DNA fragments have been separated quickly, efficiently and successfully by capillary gel electrophoresis (CGE). In this study CGE has been applied to distinguish between the discrete pattern of degraded DNA produced by apoptosis and randomized DNA breaks produced by ionizing radiation. The influence of different variables has been discussed and an example of fast separation by CGE of the apoptotic fragments produced by UV light treatment is shown.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Dano ao DNA
/
DNA
/
Apoptose
/
Eletroforese Capilar
Tipo de estudo:
Clinical_trials
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article