Effects of phytoestrogens on aromatase, 3beta and 17beta-hydroxysteroid dehydrogenase activities and human breast cancer cells.
Life Sci
; 66(14): 1281-91, 2000 Feb 25.
Article
em En
| MEDLINE
| ID: mdl-10755463
ABSTRACT
Isoflavones and others phytoestrogens have been suggested to be anticarcinogenic. Anti-aromatase, antiestrogenic or antiproliferative actions of these compounds have been postulated and related to the observation that there is a reduced incidence of breast cancer associated with diet. In this study, we explored some mechanisms by which they can exert cancer-preventive effects. Phytoestrogens were tested for estimating anti-aromatase, anti-3beta-hydroxysteroid dehydrogenase delta5/delta4 isomerase (3beta-HSD) and anti-17beta-hydroxysteroid dehydrogenase (17beta-HSD) activities in human placental microsomes. We found that isoflavonoids and compounds which presented the phenolic B ring in the 3 position on the pyran ring preferentially inhibited 3beta-HSD and/or 17beta-HSD activities than aromatase activity. We also evaluated their interactions with the estrogen receptor using a stably transfected human breast cancer cell line (MVLN). On the other hand phytoestrogens were evaluated for their effects on the proliferation in estrogen-dependent (MCF-7) and independent (MDA-MB231) human breast cancer cells. We established a relationship structure-activity and determined regions or/and substituents essential for these different activities. However, at high concentrations it seems that some phytoestrogens exert their protection against breast cancer through other estrogen-independent mechanisms.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
Pterocarpanos
/
Inibidores da Aromatase
/
Inibidores Enzimáticos
/
Estrogênios não Esteroides
/
17-Hidroxiesteroide Desidrogenases
/
3-Hidroxiesteroide Desidrogenases
Limite:
Humans
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article