Major and 5S ribosomal sequences of the largemouth bass Micropterus salmoides (Perciformes, Centrarchidae) are localized in GC-rich regions of the genome.
Chromosome Res
; 8(3): 213-8, 2000.
Article
em En
| MEDLINE
| ID: mdl-10841048
ABSTRACT
Major and 5S ribosomal genes have been localized in the chromosomes of Micropterus salmoides. By C-banding, Ag-staining, CMA3-staining and 45S and 5S fluorescence in-situ hybridization (FISH), we demonstrate that the 45S and 5S ribosomal genes are clustered in two different chromosome pairs and both are located in heterochromatic GC-rich regions. PCR amplification and sequencing of the 5S intergenic non-transcribed sequences have allowed us to identify variability essentially due to a trinucleotide tandem repeat (GCT).
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Base de dados:
MEDLINE
Assunto principal:
Bass
/
DNA Ribossômico
/
RNA Ribossômico 5S
/
Genoma
Limite:
Animals
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article