Activation of purified human T cells by mitogens: diminished mitogen-induced deoxyribonucleic acid synthesis in human T cells compared with autologous peripheral blood lymphocytes.
Infect Immun
; 13(4): 1061-8, 1976 Apr.
Article
em En
| MEDLINE
| ID: mdl-1084328
ABSTRACT
Human thymus-derived (T) cells were isolated from peripheral blood after rosette formation with neuraminidase-treated sheep erythrocytes (SRBC). After separation on Ficoll-hypaque, SRBC were removed from T cells by treatment with tris(hydroxymethyl)aminomethane-NH4Cl. Human T cells and autologous peripheral blood lymphocytes (PBL) were then incubated with phytohemagglutinin, concanavalin A, or pokeweed mitogen. Human T cells, in the absence of other cell types, responded with less deoxyribonucleic acid (DNA) synthesis (measured by uptake of [3H]thymidine) than equal numbers of autologous PBL. Further experimentation established that, compared with autologous PBL, the diminished capacity of human T cells to be activated by mitogens was due neither to differences in the mitogen dose-response relationship nor to the time of peak DNA synthesis of T cells or autologous PBL. Fragments or components of SRBC were not detected on human T cells, and treatment of the T cell-SRBC mixture with tris(hydroxymethyl)aminomethane-NH4Cl did not contribute to the results. Increased cell density or a period of preculture before addition of mitogen also did not influence the degree of decreased DNA synthesis in human T cells compared with the response of autologous PBL incubated with the same mitogens. When mixed with the cells remaining at the Ficoll-media interface, purified human T cells did not suppress the mitogenic response of this cell mixture to PHA or concanavalin A. The data indicated that human T cells, in the absence of other cell types, were activated by mitogens to a lesser degree than autologous PBL. Furthermore, T cells responded with DNA synthesis after direct T cell-mitogen interaction.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
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Ativação Linfocitária
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Linfócitos
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Linfócitos T
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Mitógenos
Limite:
Humans
Idioma:
En
Ano de publicação:
1976
Tipo de documento:
Article