Scintillation autofluorographic assessment of isotope uptake in human glioma cells grown on microtitration plates using sodium salicylate.
Anticancer Drugs
; 11(4): 243-8, 2000 Apr.
Article
em En
| MEDLINE
| ID: mdl-10898538
ABSTRACT
We describe a simple method for detecting [35S]methionine-labeled protein in fixed human astrocytoma cells grown in 96-well microtitration plates using a modified scintillation autofluorographic method. Following isotopic labeling, cells are fixed in situ and a solution of salicylic acid in methanol is dried onto the cell layer. The fluorographic image is detected using blue-sensitive X-ray film attached to the base of the plate which, following development, can be quantitated using a scanning densitometer. The relationship between cell number and optical density is linear, and there is a close correlation between the dose-response curves generated by this method and alternative isotopic detection methods and cell counting. This assay provides a suitable alternative to the use of potentially toxic scintillation fluids based on organic solvents like toluene or xylene in chemosensitivity testing of human brain tumors.
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Base de dados:
MEDLINE
Assunto principal:
Radioisótopos de Enxofre
/
Neoplasias Encefálicas
/
Salicilato de Sódio
/
Glioblastoma
/
Metionina
Limite:
Humans
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article