Comparison of rapid centrifugation assay with conventional tissue culture method for isolation of dengue 2 virus in C6/36-HT cells.
J Clin Microbiol
; 38(9): 3508-10, 2000 Sep.
Article
em En
| MEDLINE
| ID: mdl-10970418
ABSTRACT
A rapid centrifugation assay was compared with conventional tube cell culture for dengue virus isolation in both sera and autopsy samples from dengue and dengue hemorrhagic fever/dengue shock syndrome fatal cases. The rapid centrifugation assay allowed isolation of virus from 16.6% more samples than the conventional method, and it shortened the time for dengue virus detection. Finally, it allowed the isolation of dengue 2 virus in 42.8% of tissue samples from five fatal cases. Our results suggest that the rapid centrifugation assay may be useful for detection of dengue virus in clinical specimens.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Virologia
/
Centrifugação
/
Dengue Grave
/
Dengue
/
Vírus da Dengue
Tipo de estudo:
Evaluation_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article