The ER repeat protein YT521-B localizes to a novel subnuclear compartment.
J Cell Biol
; 150(5): 949-62, 2000 Sep 04.
Article
em En
| MEDLINE
| ID: mdl-10973987
ABSTRACT
The characterization of distinct subnuclear domains suggests a dynamic nuclear framework supporting gene expression and DNA replication. Here, we show that the glutamic acid/arginine-rich domain protein YT521-B localizes to a novel subnuclear structure, the YT bodies. YT bodies are dynamic compartments, which first appear at the beginning of S-phase in the cell cycle and disperse during mitosis. Furthermore, in untreated cells of the human cell line MCF7 they were undetectable and appeared only after drug- induced differentiation. YT bodies contain transcriptionally active sites and are in close contact to other subnuclear structures such as speckles and coiled bodies. YT bodies disperse upon actinomycin D treatment, whereas other transcriptional inhibitors such as alpha-amanitin or DRB have little effect. On the basis of our experiments, we propose that YT521-B may participate in the assembly of genes into transcription centers, thereby allowing efficient regulation of gene expression.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Nucleares
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Ciclo Celular
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Núcleo Celular
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Proteínas de Ligação a RNA
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Proteínas do Tecido Nervoso
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article