Identification of a novel human tRNA(Ser(CGA)) functional in murine leukemia virus replication.
Biochim Biophys Acta
; 1492(1): 264-8, 2000 Jun 21.
Article
em En
| MEDLINE
| ID: mdl-11004500
We have identified a human tRNA(Ser) isoacceptor matching the UCG codon. The tRNA was discovered via its ability to act in reverse transcription of a murine leukemia virus vector containing a complementary tRNA primer binding site (Lund et al., Nucleic Acids Res., 28 (2000) 791-799). The tRNA(Ser(CGA)) was detected in cell lines of human, monkey and mouse origin. The UCG codon is the most rarely used codon in human genes. The cloned human tRNA(Ser(CGA)) gene encodes an 85 nucleotide, intron-less tRNA, contains a consensus split intragenic promoter and is located at region p21.3-22.2 on chromosome 6. The integrity and functionality of the cloned tRNA(Ser(CGA)) gene was verified by in vitro transcription analysis in HeLa nuclear extracts.
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Base de dados:
MEDLINE
Assunto principal:
Replicação Viral
/
RNA de Transferência de Serina
/
Vírus da Leucemia Murina
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article