PHA-induced cell proliferation rescues human peripheral blood lymphocytes from X-ray-induced apoptosis.
Mutagenesis
; 16(2): 115-20, 2001 Mar.
Article
em En
| MEDLINE
| ID: mdl-11230552
ABSTRACT
Human peripheral blood G(0) lymphocytes were X-irradiated and allowed to recover for different periods both in the presence and absence of phytohemagglutinin (PHA). For each experimental condition the induction of apoptosis was investigated by nuclear morphology and formation of both DNA laddering and high molecular weight DNA fragments by pulsed field gel electrophoresis. The results showed that PHA cell growth stimulation could rescue peripheral blood lymphocytes (PBLs) from X-ray-induced apoptotic cell death. Instead, most X-irradiated lymphocytes held in G(0) phase, once they were committed to apoptosis, inexorably executed the process. These data indicate that the proliferative status of PBLs can influence apoptotic cell death PHA-stimulated PBLs appear to be more radioresistant as a result of a less efficient apoptotic process. Therefore, in current tests for mutagenicity or cytotoxicity and in biodosimetrical studies the possible role of apoptosis has to be considered.
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Base de dados:
MEDLINE
Assunto principal:
Fito-Hemaglutininas
/
Linfócitos
/
Apoptose
Limite:
Humans
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article