Detection of Borrelia burgdorferi DNA in urine of patients with ocular Lyme borreliosis.
Br J Ophthalmol
; 85(5): 552-5, 2001 May.
Article
em En
| MEDLINE
| ID: mdl-11316715
ABSTRACT
AIM:
To evaluate the diagnostic value of the polymerase chain reaction (PCR) to detect Borrelia burgdorferi DNA in patients with ocular Lyme borreliosis.METHODS:
Of 256 consecutive uveitis patients six selected individuals with clinical evidence for Lyme borreliosis and 30 patients with non-Lyme uveitis were enrolled. Lyme serology was performed by ELISA and western blotting. Urine samples were examined by an optimised nested polymerase chain reaction (PCR) protocol.RESULTS:
Only four of six uveitis patients suspected for Lyme borreliosis were ELISA positive, while all six subjects showed a positive western blot. B burgdorferi PCR was positive in all of these six patients. Whereas two of the 30 controls had a positive Lyme serology, B burgdorferi DNA was not detectable by PCR in any sample from these patients.CONCLUSIONS:
PCR for the detection of B burgdorferi DNA in urine of uveitis patients is a valuable tool to support the diagnosis of ocular Lyme borreliosis. Moreover, these patients often show a weak humoral immune response which may more sensitively be detected by immunoblotting.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA Bacteriano
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Doença de Lyme
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Grupo Borrelia Burgdorferi
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Uveíte Supurativa
Tipo de estudo:
Diagnostic_studies
/
Guideline
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Observational_studies
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Risk_factors_studies
Limite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article