Binding of tenascin-X to decorin.
FEBS Lett
; 495(1-2): 44-7, 2001 Apr 20.
Article
em En
| MEDLINE
| ID: mdl-11322944
Tenascin-X (TN-X) is an extracellular matrix protein whose absence results in an alteration of the mechanical properties of connective tissue. To understand the mechanisms of integration of TN-X in the extracellular matrix, overlay blot assays were performed on skin extracts. A 100 kDa molecule interacting with TN-X was identified by this method and this interaction was abolished when the extract was digested by chondroitinase. By solid-phase assays, we showed that dermatan sulfate chains of decorin bind to the heparin-binding site included within the fibronectin-type III domains 10 and 11 of TN-X. We thus postulate that the association of TN-X with collagen fibrils is mediated by decorin and contributes to the integrity of the extracellular network.
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Base de dados:
MEDLINE
Assunto principal:
Proteoglicanas
/
Tenascina
Limite:
Animals
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article