The Drosophila proteins Pelle and Tube induce JNK/AP-1 activity in mammalian cells.
FEBS Lett
; 497(2-3): 153-8, 2001 May 25.
Article
em En
| MEDLINE
| ID: mdl-11377431
ABSTRACT
The mammalian interleukin-1 (IL-1) signal transduction pathways display remarkable homology to the Toll signaling cascade in Drosophila. To address the question whether members of the Drosophila Toll pathway are functional in mammalian cells, inactive and constitutively active versions of the protein kinase Pelle and its regulator Tube were expressed in HeLa cells and tested for their impact on IL-1-dependent signaling events. The Drosophila proteins failed to induce the IL-1-responsive transcription factor, nuclear factor-kappaB, but selectively activated the IL-1-regulated kinase, c-Jun N-terminal kinase (JNK), thus resulting in elevated AP-1 activity. Activation of JNK/AP-1 activity was seen upon expression of a Pelle mutant lacking its C-terminal half or by a membrane-bound and multimerised Tube protein, showing the functionality of the Drosophila proteins in mammalian cells.
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Base de dados:
MEDLINE
Assunto principal:
Receptores Imunológicos
/
Interleucina-1
/
Proteínas Serina-Treonina Quinases
/
Fator de Transcrição AP-1
/
Proteínas de Insetos
/
Proteínas Quinases Ativadas por Mitógeno
/
Proteínas de Drosophila
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article