The Dictyostelium homologue of mammalian soluble adenylyl cyclase encodes a guanylyl cyclase.
EMBO J
; 20(16): 4341-8, 2001 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-11500361
ABSTRACT
A new Dictyostelium discoideum cyclase gene was identified that encodes a protein (sGC) with 35% similarity to mammalian soluble adenylyl cyclase (sAC). Gene disruption of sGC has no effect on adenylyl cyclase activity and results in a >10-fold reduction in guanylyl cyclase activity. The scg- null mutants show reduced chemotactic sensitivity and aggregate poorly under stringent conditions. With Mn(2+)/GTP as substrate, most of the sGC activity is soluble, but with the more physiological Mg(2+)/GTP the activity is detected in membranes and stimulated by GTPgammaS. Unexpectedly, orthologues of sGC and sAC are present in bacteria and vertebrates, but absent from Drosophila melanogaster, Caenorhabditis elegans, Arabidopsis thaliana and Saccharomyces cerevisiae.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Adenilil Ciclases
/
Dictyostelium
/
Guanilato Ciclase
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article