Galactosylated chitosan-graft-poly(ethylene glycol) as hepatocyte-targeting DNA carrier.
J Control Release
; 76(3): 349-62, 2001 Oct 19.
Article
em En
| MEDLINE
| ID: mdl-11578748
ABSTRACT
Lactobionic acid bearing galactose group was coupled with chitosan for liver specificity, and poly(ethylene glycol) (PEG) was grafted to galactosylated chitosan (GC) for stability in water and enhanced cell permeability. Complex formation of galactosylated chitosan-graft-PEG (GCP)/DNA complexes was confirmed by agarose gel electrophoresis. Compared to GC/DNA complex, the stability of GCP/DNA complex could be enhanced. Particle sizes of GCP/DNA complexes decreased as the charge ratio of GCP to DNA increased and had a minimum value around 27 nm at the charge ratio of 5. Conformational change of DNA did not occur after complex formation with GCP compared to conformation of DNA itself. GCP/DNA complexes were only transfected into Hep G2 having asialoglycoprotein receptors (ASGR), indicative of specific interaction of ASGR on cells and galactose ligands on GCP.
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Base de dados:
MEDLINE
Assunto principal:
Polietilenoglicóis
/
DNA
/
Quitina
/
Hepatócitos
Limite:
Humans
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article