Tumour-associated antigen (TAA)-specific cytotoxic T cell (CTL) response in vitro and in a mouse model, induced by TAA-plasmids delivered by influenza virosomes.
Eur J Cancer
; 37(16): 2097-103, 2001 Nov.
Article
em En
| MEDLINE
| ID: mdl-11597390
ABSTRACT
We investigated influenza virosomes as a TAA-gene delivery system for use in TAA-directed anti-cancer vaccine therapy. An engineered plasmid (GC90) expressing the parathyroid hormone-related peptide (PTH-rP), a protein secreted by prostate and lung carcinoma cells, was included in influenza virosomes (GC90V). The ability of GC90V to elicit a PTH-rP-specific cytotoxic T cell (CTL) response was demonstrated in BALB/c mice immunised with intranasal (i.n.) GC90V+/-adjuvant subcutaneous (s.c.) interleukin-2 (IL-2). A PTH-rP-specific CTL response with antitumour activity was also demonstrated in human peripheral blood mononuclear cells (PBMC) stimulated in vitro with GC90V infected autologous dendritic cells (DC). These results provide a rationale for investigating GC90V in clinical trials of anticancer vaccine therapy.
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos T Citotóxicos
/
Técnicas de Transferência de Genes
/
Vacinas Anticâncer
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Citotoxicidade Imunológica
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Antígenos de Neoplasias
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Limite:
Animals
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Female
/
Humans
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Male
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article