Mitogenic effect of gastrin and expression of gastrin receptors in duct-like cells of rat pancreas.
Gastroenterology
; 121(4): 940-9, 2001 Oct.
Article
em En
| MEDLINE
| ID: mdl-11606507
ABSTRACT
BACKGROUND & AIMS:
Ductular metaplastic cells are observed during pancreas injury. Growth control by gastrin and expression of gastrin/cholecystokinin (CCK) B receptors were evaluated in these cells.METHODS:
Acinoductal transdifferentiation was induced in vitro by culturing of acinar cells, and ductular metaplasia was obtained in vivo by ligation of the pancreatic ducts. Mitogenic effects of gastrin I on ductal complexes in vivo and of tetragastrin, pentagastrin, and gastrin I and II, with or without the CCK-B receptor antagonist L-365,260, on duct-like cells in vitro were analyzed by 5-bromo-2'-deoxyuridine labeling. Immunocytochemistry, Western blotting, and reverse-transcription polymerase chain reaction were applied for detection of the CCK-B receptor.RESULTS:
Gastrin analogues induced a mitogenic stimulus in the duct-like cells in vitro and in ductal complexes in duct-ligated rat pancreas. Immunocytochemistry showed expression of CCK-B receptors in these models and in fetal but not normal adult exocrine pancreas. Additionally, up-regulation of CCK-B receptors during ductular metaplasia was shown by Western blotting and reverse-transcription polymerase chain reaction.CONCLUSIONS:
Duct-like pancreatic epithelial cells in vitro and ductal complexes in vivo express gastrin/CCK-B receptors and proliferate in response to gastrin.
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Base de dados:
MEDLINE
Assunto principal:
Ductos Pancreáticos
/
Gastrinas
/
Receptores da Colecistocinina
/
Substâncias de Crescimento
Limite:
Animals
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article