Ras-activated endocytosis is mediated by the Rab5 guanine nucleotide exchange activity of RIN1.
Dev Cell
; 1(1): 73-82, 2001 Jul.
Article
em En
| MEDLINE
| ID: mdl-11703925
ABSTRACT
RIN1 was originally identified by its ability to inhibit activated Ras and likely participates in multiple signaling pathways because it binds c-ABL and 14-3-3 proteins, in addition to Ras. RIN1 also contains a region homologous to the catalytic domain of Vps9p-like Rab guanine nucleotide exchange factors (GEFs). Here, we show that this region is necessary and sufficient for RIN1 interaction with the GDP-bound Rabs, Vps21p, and Rab5A. RIN1 is also shown to stimulate Rab5 guanine nucleotide exchange, Rab5A-dependent endosome fusion, and EGF receptor-mediated endocytosis. The stimulatory effect of RIN1 on all three of these processes is potentiated by activated Ras. We conclude that Ras-activated endocytosis is facilitated, in part, by the ability of Ras to directly regulate the Rab5 nucleotide exchange activity of RIN1.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Transporte
/
Proteínas ras
/
Proteínas rab de Ligação ao GTP
/
Proteínas rab5 de Ligação ao GTP
/
Proteínas de Saccharomyces cerevisiae
/
Proteínas de Transporte Vesicular
/
Endocitose
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article