TNF-alpha modulates cytokine and cytokine receptors in C2C12 myotubes.
Cancer Lett
; 175(2): 181-5, 2002 Jan 25.
Article
em En
| MEDLINE
| ID: mdl-11741746
ABSTRACT
Incubation of C2C12 differentiated myotubes in the presence of 1000 units/ml (100 ng/ml) of recombinant murine tumor necrosis factor-alpha (TNF-alpha) for up 6 h caused an important increase in mRNA content of the cytokine (10-fold at 6 h). The levels of expression of the two cytokine receptors were not changed by the cytokine at short-time intervals, but 48 h of treatment resulted in a decreased expression of TNFR1 (33%). Interestingly, the presence of the cytokine resulted in significant increases in mRNA content for the catabolic pro-inflammatory cytokines IL-6 (17-fold) and IFN-gamma (8-fold). Similarly, TNF-alpha also caused a moderate increased expression of different anti-inflammatory cytokines such as IL-9 (104%), IL-10 (24%) and IL-15 (47%). The results suggest that other cytokines may be involved in mediating TNF-alpha action in skeletal muscle and that anti-inflammatory cytokines may be released as a counter-regulatory mechanism.
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Base de dados:
MEDLINE
Assunto principal:
Antígenos CD
/
Regulação da Expressão Gênica
/
Citocinas
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Fator de Necrose Tumoral alfa
/
Receptores do Fator de Necrose Tumoral
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Receptores de Citocinas
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Músculo Esquelético
Limite:
Animals
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article