Comparison of real-time, quantitative PCR with molecular beacons to nested PCR and culture methods for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples.
J Clin Microbiol
; 40(1): 287-91, 2002 Jan.
Article
em En
| MEDLINE
| ID: mdl-11773135
ABSTRACT
An automated PCR with fluorescent probes (molecular beacons) detected Mycobacterium avium subsp. paratuberculosis in bovine feces. When the PCR was compared with culture in testing 41 fecal samples, kappa scores of 0.94 to 0.96, a sensitivity of 93 to 96%, and a specificity of 92% were obtained. Results were quantitated by using a standard curve derived from a plasmid containing IS900. A minimum quantity of 1.7 x 10(-4) pg of DNA, correlating to 1 to 8 CFU, was detected.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Paratuberculose
/
Doenças dos Bovinos
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Reação em Cadeia da Polimerase
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Mycobacterium avium subsp. paratuberculosis
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Fezes
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article