Import of adenovirus DNA involves the nuclear pore complex receptor CAN/Nup214 and histone H1.
Nat Cell Biol
; 3(12): 1092-100, 2001 Dec.
Article
em En
| MEDLINE
| ID: mdl-11781571
ABSTRACT
Adenovirus type 2 (Ad2) imports its DNA genome through the nuclear pore complex (NPC) of cells in interphase for viral production. Here we identify the NPC-filament protein CAN/Nup214 as a docking site for incoming Ad2 capsids. Binding to CAN is independent of cytosolic factors. Capsids disassemble at NPCs to free their DNA for import. This process requires binding of nuclear histone H1 to the stably docked capsids and involves H1-import factors, restricting this irreversible process to the proximity of the nucleus. Our results provide a molecular mechanism for disassembly of Ad2 and reveal an unexpected function of histone H1 in virus-mediated DNA import.
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Base de dados:
MEDLINE
Assunto principal:
DNA Viral
/
Histonas
/
Adenoviridae
/
Poro Nuclear
/
Complexo de Proteínas Formadoras de Poros Nucleares
/
Proteínas do Capsídeo
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article