Differentiation of Trypanosoma rangeli: high production of infective Trypomastigote forms in vitro.
Parasitol Res
; 88(1): 21-5, 2002 Jan.
Article
em En
| MEDLINE
| ID: mdl-11822733
ABSTRACT
In the present study, we report a simple method to induce high Trypanosoma rangeli differentiation in vitro, producing a large number of infective trypomastigote forms. Parasites from SC-58 (Brazil) and Choachi (Colombia) strains were cultivated at 27 degrees C in TC-100, Grace and DMEM media, each supplemented with 5% fetal bovine serum and prepared at three distinct pHs (6.0, 7.0, 8.0). Differentiation was microscopically evaluated at 0, 3 and 6 days after cultivation in each medium by determining the percentage of trypomastigotes in Giemsa-stained smears. Our data revealed similar results for both T. rangeli strains, showing (after 6 days of cultivation in DMEM medium, pH 8.0) the presence of about 80% of trypomastigotes. These culture-derived trypomastigotes proved to be infective to both Balb-C mice and Rhodnius spp, reaching the triatomine's salivary glands. Our results describe a new and easy method to induce high T. rangeli differentiation in vitro, allowing further studies on the antigenic constitution of trypomastigotes.
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Base de dados:
MEDLINE
Assunto principal:
Parasitologia
/
Trypanosoma
Limite:
Animals
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article