[Effect of glycogen on calcium of donor liver during ischemia-reperfusion period].
Zhonghua Gan Zang Bing Za Zhi
; 10(1): 21-4, 2002 Feb.
Article
em Zh
| MEDLINE
| ID: mdl-11856494
ABSTRACT
OBJECTIVE:
To evaluate the effect of glycogen on calcium concentration of rabbit donor liver during ischemia-reperfusion period.METHODS:
Donor group (n=21) was divided into 3 subgroups randomly Group A (n=7) fasting for 24 hours before harvesting; Group B (n=7) normal laboratory chew; Group C (n=7) normal laboratory chew plus glucose supplement intravenously. Based on the self-created animal model for ischemia-reperfusion, the levels of glycogen content, ATP level, viability of Ca(2+)ATPase and plasmic free Ca(2+) concentration ([Ca(2+)]i) of liver tissue were measured.RESULTS:
Before cold preservation, there was a significant difference of glycogen content among the three groups at all time points except at the end of rewarming period. ATP level and Ca(2+)ATPase viability were significantly higher in group C than in other two groups. But the plasmic free Ca(2+) concentration was lower in groups with higher glycogen content.CONCLUSIONS:
Donor liver with high glycogen content can provide relatively sufficient ATP, maintain better Ca(2+)ATPase viability and prevent plasmic free Ca(2+) concentration overloading. This maybe an important mechanism for glycogen to ameliorate ischemia-reperfusion injury to the donor livers.
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Base de dados:
MEDLINE
Assunto principal:
Traumatismo por Reperfusão
/
Trifosfato de Adenosina
/
Cálcio
/
Transplante de Fígado
/
ATPases Transportadoras de Cálcio
/
Glicogênio
/
Hepatopatias
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2002
Tipo de documento:
Article