B cell function after haploidentical in utero bone marrow transplantation in a patient with severe combined immunodeficiency.
Bone Marrow Transplant
; 29(7): 625-8, 2002 Apr.
Article
em En
| MEDLINE
| ID: mdl-11979315
ABSTRACT
An in utero paternal CD34(+) cell transplant was performed in a T-B+NK+ SCID fetus. We report here the results of the 3-year humoral immune reconstitution study. The methods used were ApoB VNTR typing, flow cytometry, nephelometry, hemagglutination, ELISA, ELISPOT and lymphoproliferative assays. The T cells were of donor origin whereas monocytes, B and NK cells were of host origin. Peripheral B cell counts and IgM levels were normal since birth. IVIG therapy was required at 5 months of age until 2 years old. IgA levels > or =20 mg/dl were detected from month 17 post transplantation. Isohemagglutinins were present since month 8 post transplantation, the highest titers (anti-A1/128, anti-B1/32) were obtained at month 33 post-transplantation. After immunization with rHBsAg, circulating anti-HBsAg IgG secreting cells and a 7.8-fold increase in serum anti-HBsAg Ab were detected. We conclude that split chimerism following in utero haploidentical BMT allows complete humoral immune reconstitution in a T-B+NK+ SCID patient.
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
/
Transplante de Medula Óssea
/
Imunodeficiência Combinada Severa
/
Quimeras de Transplante
/
Doenças Fetais
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
/
Prognostic_studies
Limite:
Humans
/
Male
/
Newborn
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article