Effect of pH and Ca2+-induced associations of soybean proteins.
J Agric Food Chem
; 50(17): 4953-8, 2002 Aug 14.
Article
em En
| MEDLINE
| ID: mdl-12166988
ABSTRACT
An experimental procedure was developed to characterize the solubility of the soybean protein fractions close to the isoelectric point. The results show that the 7S fraction is precipitated in a much narrower range of pH values than the 11S fraction. Surprisingly, the addition of salt to the solutions leads to increased solubility of proteins, unlike the common "salting out" effect generally expected for proteins in solution in this range of salt concentrations. The precipitation equilibria of both soybean fractions in the presence of calcium ions and electrolyte were characterized. The amount of calcium ions required to precipitate a mole of the 7S fraction is much larger than that required for the 11S fractions. The precipitation pattern can be correlated to the charge density per surface area of the proteins.
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Base de dados:
MEDLINE
Assunto principal:
Cálcio
/
Proteínas de Soja
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article