[Construction of eukaryotic expression vector using neomycin-resistance gene mutant as selectable marker].
Sheng Wu Gong Cheng Xue Bao
; 18(3): 308-12, 2002 May.
Article
em Zh
| MEDLINE
| ID: mdl-12192863
ABSTRACT
Neomycin-resistance gene is widely used as a selectable marker in eukaryotic expression vector. It codes neomycin phosphotransferase II (NPT II) which confers resistance to various aminoglycoside antibiotic such as G418 and kanamycine. In this work, by site-directed mutagenesis the neo gene mutant was obtained. The expression vector pmDNA using the neo gene mutant as selectable marker has been constructed. After inserting interest luciferase gene, the expression plasmid pmDNAluc + was stably transfected CHO-K1 cells. As a result, the expression positive ratio reaches to approximate 95% and the ratio of high expression colonies is apparently higher than the controls.
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Base de dados:
MEDLINE
Assunto principal:
Resistência a Medicamentos
/
Marcadores Genéticos
/
Canamicina Quinase
/
Vetores Genéticos
Idioma:
Zh
Ano de publicação:
2002
Tipo de documento:
Article