Your browser doesn't support javascript.
loading
Effects of sterigmatocystin, deoxynivalenol and aflatoxin G1 on apoptosis of human peripheral blood lymphocytes in vitro.
Sun, Xu-Ming; Zhang, Xiang-Hong; Wang, Hui-Yan; Cao, Wen-Jun; Yan, Xia; Zuo, Lian-Fu; Wang, Jun-Ling; Wang, Feng-Rong.
Afiliação
  • Sun XM; Department of Experimental Pathology, Hebei Medical University, Shijiazhuang 050017, Hebei, China.
Biomed Environ Sci ; 15(2): 145-52, 2002 Jun.
Article em En | MEDLINE | ID: mdl-12244755
ABSTRACT

OBJECTIVE:

To explore the effects of Sterigmatocystin (ST), Deoxynivalenol (DON) and Aflatoxin G1 (AFG1) on apoptosis of human peripheral blood lymphocytes (HPBLs) in vitro and thus to further elucidate the putative roles of these three mycotoxins on human immunosystem.

METHODS:

The effects of ST, DON and AFG1 on apoptosis of HPBLs were studied with cell culture, flow cytometric (FCM) DNA analysis and DNA agarose gel electrophoresis.

RESULTS:

DNA agarose gel electrophoresis results showed the characteristic "ladder" pattern of apoptosis in HPBLs treated with ST, DON and AFG1. Flow cytometric DNA analysis revealed that typical subdiploid peaks of apoptosis in DNA histogram could be seen in all groups treated with the three mycotoxins. Significant time-effect and dose-effect relationships were found between the apoptosis rates and treatment time as well as concentrations of the three mycotoxins.

CONCLUSION:

ST, DON and AFG1 can induce apoptosis of HPBLs in vitro and may have some negative effects on human immunosystem.
Assuntos
Buscar no Google
Base de dados: MEDLINE Assunto principal: Esterigmatocistina / Tricotecenos / Linfócitos / Apoptose / Aflatoxinas Limite: Humans Idioma: En Ano de publicação: 2002 Tipo de documento: Article
Buscar no Google
Base de dados: MEDLINE Assunto principal: Esterigmatocistina / Tricotecenos / Linfócitos / Apoptose / Aflatoxinas Limite: Humans Idioma: En Ano de publicação: 2002 Tipo de documento: Article