HL-60 cell growth-conditioned medium is an effective inducer of myeloperoxidase expression in K-562 human leukemia cells.
Leuk Res
; 26(11): 1017-25, 2002 Nov.
Article
em En
| MEDLINE
| ID: mdl-12363471
ABSTRACT
K-562 cells were cultured in HL-60 cell growth-conditioned medium (GCM) for up to 96h. Myeloperoxidase (MPO) mRNA was transiently detected by reverse transcription-polymerase chain reaction (RT-PCR) techniques at 12, 24, and 48h. The de novo expression of MPO protein was subsequently detectable by intracellular flow cytometry at 24, 48, 72 and 96h. Immunogold staining and cytochemical analysis demonstrated granularly-sequestered MPO in approximately 40% of HL-60 GCM-cultured cells after 48h of culture. The sequential detection of MPO mRNA and MPO biosynthesis is considered an indicator of serial maturation evocative of myeloblastic cells, and suggest that K-562 cells maintain the ability to differentiate along this lineage.
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Base de dados:
MEDLINE
Assunto principal:
Meios de Cultivo Condicionados
/
Peroxidase
/
Células HL-60
/
Células K562
Limite:
Humans
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article