[Construction of a temperature inducible shuttle expression vector and its application in Streptomyces].
Sheng Wu Gong Cheng Xue Bao
; 18(4): 420-3, 2002 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-12385236
ABSTRACT
pHZ1080, an E. coli-Streptomyces shuttle expression vector was constructed in order to explore the utilization of lambda phage regulated expression elements in Streptomyces. A 2.7 kb polyketide synthase (PKS) gene from Streptomyces sp. FR-008 was inserted into downstream of lambda phage promoter (PR) to give the shuttle plasmid, pHZ1067. The PKS protein was expressed in Streptomyces lividans carrying pHZ1067 in a heat-dependent manner, as it did in E. coli. The PKS protein expressed in both hosts with same molecular weight was detected by SDS-PAGE and Western-blot. The successful heat-induced expression of PKS suggested that pHZ1080 was useful and convenient for heat-induced expression of heterologous genes in both E. coli and Streptomyces.
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Base de dados:
MEDLINE
Assunto principal:
Streptomyces
/
Vetores Genéticos
Idioma:
Zh
Ano de publicação:
2002
Tipo de documento:
Article