Sensitive biomonitoring of monoterpene exposure by gas chromatographic-mass spectrometric measurement of hydroxy terpenes in urine.
J Chromatogr B Analyt Technol Biomed Life Sci
; 780(2): 225-30, 2002 Nov 25.
Article
em En
| MEDLINE
| ID: mdl-12401347
ABSTRACT
A gas chromatographic method with mass selective detection was developed which enables the simultaneous determination of the urinary hydroxy terpenes cis-verbenol, alpha-terpineol, myrtenol, carveol, perillyl alcohol and trans-sobrerol. The sample preparation consisted of enzymatic hydrolysis, solid phase extraction (SPE) with RP-C(18) SPE material and clean up with silica gel cartridges. Large volume injection was used and the mass selective detection was done in the single ion modus. Low detection limits in the range of 1.0-4.5 microg/l for the terpene metabolites in urine and mean recoveries of 100% were achieved.
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Base de dados:
MEDLINE
Assunto principal:
Monitoramento Ambiental
/
Monoterpenos
/
Cromatografia Gasosa-Espectrometria de Massas
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article