Mapping of cyclosporin A binding sites in cyclophilin A by using synthetic peptides.
Regul Pept
; 59(1): 23-30, 1995 Sep 22.
Article
em En
| MEDLINE
| ID: mdl-12506411
ABSTRACT
In order to map cyclosporin A (CsA) binding sites of cyclophilin (CyP), we synthesized the complete set of overlapping 157 octapeptides corresponding to human CyP A using the multi-pin peptide synthesis system. The pin-coupled synthetic octapeptides were examined in terms of binding ability to CsA by a modification of the enzyme-linked immunosorbent assay. Significant binding of CsA was detected with 35 synthetic N alpha-acetylated octapeptides possessing the N-terminal amino acids corresponding to the residues in positions 24-26, 42-44, 69-73, 75, 76, 89-91, 102, 116, 124-131, 144-151 and 152 in human CyP A, respectively. Other eight octapeptides showed moderate CsA binding activity. The distinct binding of octapeptides covering the C-terminal region of the CyP A was particularly significant. These data are to be compared with the information provided by X-ray and NMR studies on the CsA binding sites and furnish thus a test of the reported method. The present study also gave added insight into the CsA interaction sites of CyP.
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Base de dados:
MEDLINE
Assunto principal:
Ciclosporina
/
Ciclofilina A
Limite:
Humans
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article