Dissecting apicoplast targeting in the malaria parasite Plasmodium falciparum.
Science
; 299(5607): 705-8, 2003 Jan 31.
Article
em En
| MEDLINE
| ID: mdl-12560551
ABSTRACT
Transit peptides mediate protein targeting into plastids and are only poorly understood. We extracted amino acid features from transit peptides that target proteins to the relict plastid (apicoplast) of malaria parasites. Based on these amino acid characteristics, we identified 466 putative apicoplast proteins in the Plasmodium falciparum genome. Altering the specific charge characteristics in a model transit peptide by site-directed mutagenesis severely disrupted organellar targeting in vivo. Similarly, putative Hsp70 (DnaK) binding sites present in the transit peptide proved to be important for correct targeting.
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Base de dados:
MEDLINE
Assunto principal:
Plasmodium falciparum
/
Sinais Direcionadores de Proteínas
/
Organelas
/
Proteínas de Protozoários
/
Transporte Proteico
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article