A procedure for pulmonary lavage in mice.
Am J Vet Res
; 37(2): 237-8, 1976 Feb.
Article
em En
| MEDLINE
| ID: mdl-1259218
ABSTRACT
A method for the pulmonary lavage of mice is described. The procedure includes exsanguination of anesthetized mice by severting the renal artery, inserting a tracheal catheter in situ, and repeatedly injecting and aspirating 0.9% sodium chloride solution. Protein was recovered from the cell-free lavage fluid even after a given mouse was lavaged several times. The major part of the protein, however, was obtained with 1-ml washes repeated 3 times. Approximately 0.563 mg of protein was recovered by the procedure from a 29-g mouse. Four lavages per mouse yielded approximately 2.9 x 106 free cells.
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Base de dados:
MEDLINE
Assunto principal:
Irrigação Terapêutica
/
Pulmão
Limite:
Animals
Idioma:
En
Ano de publicação:
1976
Tipo de documento:
Article