[Identification of orthopoxvirus species using oligonucleotide micro-chips]. / Vidovaia identifikatsiia ortopoksvirusov na oligonukleotidnykh mikrochipakh.
Vopr Virusol
; 48(1): 4-9, 2003.
Article
em Ru
| MEDLINE
| ID: mdl-12608052
ABSTRACT
A method for describing the Orthopoxviruses that are pathogenic both to man and animals is described in the article. The method is based on hybridization of a fluorescently labelled amplified DNA sample with oligonucleotides, which were immobilized in a microchip. Species-specific regions within the crmB gene encoding a viral analogue of the tumor necrosis factor receptor, i.e. an important gene determining the pathogenicity of the mentioned Orthopoxviruses type, were used as a target for identification. The identification procedure takes around 6 hours and does not demand any costly equipment (a portable fluorescent microscope can be used).
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
Receptores do Fator de Necrose Tumoral
/
Orthopoxvirus
/
Análise de Sequência com Séries de Oligonucleotídeos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
Ru
Ano de publicação:
2003
Tipo de documento:
Article