Effects of icariin on cGMP-specific PDE5 and cAMP-specific PDE4 activities.
Asian J Androl
; 5(1): 15-8, 2003 Mar.
Article
em En
| MEDLINE
| ID: mdl-12646997
ABSTRACT
AIM:
To clarify the mechanism of the therapeutic action of icariin on erectile dysfunction (ED).METHODS:
PDE5 was isolated from the human platelet and PDE4 from the rat liver tissue using the FPLC system (Pharmacia, Milton Keynes, UK) and the Mono Q column. The inhibitory effects of icariin on PDE5 and PDE4 activities were investigated by the two-step radioisotope procedure with [(3)H]-cGMP/[(3)H]-cAMP. Papaverine served as the control drug.RESULTS:
Icariin and papaverine showed dose-dependent inhibitory effects on PDE5 and PDE4 activities. The IC(50) of Icariin and papaverine on PDE5 were 0.432 micromol/L and 0.680 micromol/L, respectively and those on PDE4, 73.50 micromol/L and 3.07 micromol/L, respectively. The potencies of selectivity of icariin and papaverine on PDE5 (PDE4/PDE5 of IC(50)) were 167.67 times and 4.54 times, respectively.CONCLUSION:
Icariin is a cGMP-specific PDE5 inhibitor that may be developed into an oral effective agent for the treatment of ED.
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Base de dados:
MEDLINE
Assunto principal:
Flavonoides
/
Ereção Peniana
/
Medicamentos de Ervas Chinesas
/
3',5'-GMP Cíclico Fosfodiesterases
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article