Targeting the MraY and MurG bacterial enzymes for antimicrobial therapeutic intervention.
Anal Biochem
; 314(2): 243-52, 2003 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-12654311
ABSTRACT
Assays for two enzymes from Escherichia coli were developed and validated as antibacterial inhibitor screens. The MraY and MurG enzymes were overexpressed and purified as the membrane fraction or to homogeneity, respectively. The MurG enzyme was expressed with a six-histidine tag using an optimized minimal-medium protocol for subsequent purification. Although traditional assays were established, the enzymes were also assayed via a 96-well membrane plate assay and a 384-well scintillation proximity-based assay developed herein. These assays afford a more economical and high-throughput evaluation of inhibitors. A mureidomycin inhibitor mix was used as a control for the assay development and screen validation. Several inhibitors resulting from a high-throughput screen were found and evaluated for potential therapeutic use.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas da Membrana Bacteriana Externa
/
Proteínas de Bactérias
/
Transferases
/
N-Acetilglucosaminiltransferases
/
Proteínas de Escherichia coli
/
Escherichia coli
Tipo de estudo:
Guideline
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article