[Expression of porcine interferon-gamma gene in Pichia pastoris and its effect of inhibiting porcine reproductive and respiratory syndrome virus].
Sheng Wu Gong Cheng Xue Bao
; 18(6): 683-6, 2002 Nov.
Article
em Zh
| MEDLINE
| ID: mdl-12674637
ABSTRACT
In order to develop recombinant porcine Interferon-gamma (rPoIFN-gamma) to prevent porcine viral infection, PoIFN-gamma cDNA lacking the signal peptide was expressed in Pichia pastoris GS115 strain, and the effect of rPoIFN-gamma on porcine reproductive and respiratory syndrome virus (PRRSV) was investigated. The PoIFN-gamma gene was inserted into integrative vector pHIL-S1, and the recombinant GS115 strain (pHIL-S1/PoIFN-gamma) was constructed by homologues recombinant. The rPoIFN-gamma protein was 18 kD with an expressing yield of 18% was assayed by SDS-PAGE and Western blot, respectively. The anti-viral activity of rPoIFN-gamma was in the range of 450-540 u/mL. In addition, the effect of rPoIFN-gamma ant-PRRSV was determined using CPE50 method. The results indicated that high concentration of rPoIFN-gamma could inhibit PRRSV on Marc-145 cell line. The rPoIFN-gamma is a potential drug for prevention and treatment of various kinds of viral pig diseases.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Antivirais
/
Pichia
/
Interferon gama
/
Vírus da Síndrome Respiratória e Reprodutiva Suína
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2002
Tipo de documento:
Article